The effect of various doses of an exogenous acid protease on the fermentation and nutritive value of corn silage

Abstract

The objective of this experiment was to evaluate the effects of treating whole-plant corn at harvest with various doses of an exogenous acidic protease on fermentation and changes in nutritive value after a short period (45 d) of ensiling. Whole-plant corn (37% dry matter) was chopped and treated with 0, 20, 200, 1,000, or 2,000 mg of protease/kg of wet forage. Forages (~500 g) were packed in bag silos and ensiled at 22 to 23°C for 45 d. Data were analyzed as a 5 × 2 factorial arrangement of treatments with the main effects of the dose of protease, day of ensiling, and their interaction. Treatment with protease did not alter the concentrations of dry matter, neutral detergent fiber, acid detergent fiber, starch, lactic acid, or acetic acid compared with untreated silage, with the exception that the concentration of starch was lower in silage treated with 20 mg of protease/kg compared with untreated silage. However, the 2 highest doses of protease resulted in silages with higher concentrations of ethanol and more yeasts compared with untreated silage. Protease treatment did not affect the ruminal in vitro digestibility of neutral detergent fiber. Concentrations of soluble protein (percentage of crude protein) increased after ensiling for all treatments but was not different between silage treated with the lowest dose of protease and untreated silage. Soluble protein increased in a dose-dependent manner above the low dose of protease in silages. Concentrations of NH3-N were higher only in silages treated with the 2 highest doses of protease compared with untreated silage. Silages treated with the 3 highest doses of protease were higher in ruminal in vitro digestibility of starch compared with untreated silage but were similar to each other. The concentrations of total AA were determined in fresh forage and silages for the untreated and 200 and 2,000 mg/kg doses of protease. Neither amount of added protease affected the total concentrations of essential, nonessential, or total AA in silage. However, of the essential AA, treatment with protease resulted in silages with lower concentrations of lysine and arginine but higher concentrations of leucine compared with untreated silage. The 200 mg/kg dose of protease substantially improved ruminal in vitro starch digestion in corn silage after a short period of ensiling without affecting concentrations or numbers of ethanol and yeasts, respectively.