Development and characterization of solvent-based 3D printed polylactic acid/45S5 bioactive glass composites for soft and hard tissue engineering.

Abstract

With the benefit of offering hydrolysis breakdown and bio-resorption of its products, polylactic acid (PLA) is among the most frequently utilized polymers for many biomedical applications. Composites made of polylactic acid (PLA) and bioactive substances such as bioactive glass (BG) are developing as novel biomaterials because they comprise the mechanical properties and bioactivity of bioactive glass (BG) with the conformability and bio absorption of PLA. In this work, composites of PLA/BG were produced by employing the solvent-based three-dimensional printing process. To accomplish this, 0-2 wt% of BG particles (size ≤ 2 µm) were added to PLA. The resulting composite mix was then fed into a solvent-based 3D printer for the layer-by-layer construction of composites. According to the SEM/EDX investigation, BG particles were evenly dispersed throughout the polymer matrix which resulted in the interfacial bonding between them. FTIR and XRD analysis showed that PLA and BG did not interact chemically. All the composites were evaluated for cytocompatibility by in vitro cellular tests, which also proved their suitability as a substrate for NIH 3T3 cell adherence and growth. The composites were also found to be good in terms of hemocompatibility and platelet adhesion. In conclusion, additional studies on these materials were encouraged by the successful outcomes, which suggested that 3D-printed composite scaffolds consisting of PLA and BG particles might be useful in soft and hard tissue engineering.