Abstract

This chapter presents the development and application of microbial sensor system for determination of biochemical oxygen demand (BOD). BOD is a widely used parameter for the determination of biodegradable organic compounds in waste water. A more rapid estimation of BOD is possible by using a microbial sensor containing whole cells immobilized on an oxygen electrode. Activated sludge obtained from waste water treatment plants that contain a variety of species of microorganism population is not suitable to prepare sensors. A microbial amperometric sensor for the determination of the biochemical oxygen demand (BOD) determination using Trichosporon cutaneum cells immobilized in polyvinylalcohol has been developed. The oxygen demand of a sample is measured in the conventional test, during five days reflecting the various metabolic reactions of a mixed population. However, the determination of BOD with a microbial sensor is a test with a selected microbial species. The incubation procedure of the microbial sensor was applied to the estimation of BOD for wastewater from the food and chemical Industry.

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