Development and application of a high accuracy method for measuring Pb in blood

Abstract

BackgroundLead (Pb) is an environmental pollutant, and human exposure is assessed by measuring blood Pb concentrations. Today, the use of Pb-based products is restricted, but the health effects of low Pb concentrations require investigation. For this, a high-accuracy method is needed. Here, we report a new inductively coupled plasma mass spectrometry (ICP-MS) method for quantifying Pb in blood. MethodsBlood samples and calibrators were gravimetrically spiked with bismuth as an internal standard, digested with ultrapure nitric acid, and diluted 100 times. The calibrators were made from high-purity reference materials supplied by the National Institute of Standards and Technology, and the sample concentration was measured using the calibration bracketing method. The analytical performance and application of the new method were investigated. ResultsThe method had good specificity and a negligible matrix effect. The intra- and inter-assay coefficients of variation (CVs) were <1.34 % and <1.88 %, respectively, and the total CV was <1.74 %. The range of recovery was 98.9 %–99.4 %. SRM955d, BCR-635, and BCR-636 were within the certified intervals, with mean relative bias values of 0.93 %, −0.13 %, and −0.56 %, respectively. The limit of quantification was 1.1 µg/l. The method was used to assign target values to external quality assessment samples and to assess routine methods, showing good results at high concentrations. However, at low concentrations, two laboratories did not pass. ConclusionsThis ICP-MS method is a simple, accurate method and can be a candidate reference method for blood Pb measurement. But more research will be needed to verify this.