Abstract
The extraction of high quality genomic DNA is pivotal for all downstream genetic analyses. To assist laboratories in the quality assurance of their DNA isolation procedure, the RCPAQAP Molecular Genetics discipline developed a DNA extraction proficiency testing program in 2016 and invited six laboratories across Australia to participate in the first trial. A total of 40 DNA extracts from various tissue types were submitted to the RCPAQAP for external quality assessment. Each DNA extract was analysed on a NanoDrop Spectrophotometer (Thermo Fisher Scientific) and a Quantus Fluorometer (Promega) for concentration and purity determination, and on a DNA TapeStation (Agilent Technologies) for total DNA integrity characterisation. Evaluation of each DNA extract was based on the DNA Integrity Number (DIN), generated by DNA TapeStation, and was used for population Z-score analysis. DNA extracts with a Z-score within two standard deviations of the mean data value were considered good quality. An overview of the laboratory performance in genomic DNA extraction and the associated methods will be presented.
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