Abstract
ABSTRACTTilmicosin has been widely used in the dairy industry, but this compound is hazardous to human health. Therefore, it is urgent to develop a rapid, convenient, sensitive, cost-effective, and user-friendly method to determine tilmicosin. A fluorescent microsphere-based immunochromatographic assay was developed for this analyte. A 200-nm red fluorescent microspheres were selected to be the label, and the mass of the labeled antibody was 4.48 µg/mL. 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide was used to catalyze carboxyl groups on the surface of the microspheres. The optimum reaction time between the antibodies and fluorescent microspheres was 30 min, and under these conditions, high coupling ratios and stable conjugations were obtained. The linear dynamic range of the developed protocol was from 0.02 to 2.10 µg/L. The half maximal inhibitory concentration was 0.19 µg/L. The recoveries were from 87 to 98% with a coefficient of variation less than 7.14%. In comparison with a colloidal gold immunochromatographic assay, the developed method offered a higher coupling rate, lower amounts of antibodies, and higher sensitivity. This approach is simple, sensitive, and suitable for on-site applications on farms for rapid screening.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
