Abstract
A high-performance liquid chromatographic method was developed for monitoring the serum concentration of streptomycin. The method includes clean-up using a Sep-Pak C 18 cartridge and quantitation using dihydrostreptomycin as an internal standard. Streptomycin and dihydrostreptomycin were separated by reversed-phase ion-pair chromatography on LiChrosorb RP-18 and detected by UV absorption (195 nm). The calibration graph of serum streptomycin concentration was linear over the range 5–50 μg/ml. Streptomycin was added to serum at the level of 20.0 μg/ml and its concentration was determined to be 18.9 μg/ml with a coefficient of variation of 2.07% ( n = 5). The clinical application of this method was confirmed by comparison with fluorescence polarization immunoassay.
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