Abstract
A rapid and simple method was developed for the determination of scutellarin in Scutellaria barbata plant extracts by reversed‐phase liquid chromatography with an electrochemical detector (ECD). The potential electrode voltage was +0.7 V for the ECD. The mobile phase consisted of methanol:water:glacial acetic acid (30:68:2, v/v/v) (pH 2.7). The linear calibration ranged from 2.3 to 150.0 µg mL−1 for scutellarin. The detection limit was 0.2 µg mL−1 given a signal‐to‐noise ratio (S/N) of 3:1 on the second detection fullscale (500 nA). The six different extracts of S. barbata were determined using different extraction methods and extraction solvents. The contents of scutellarin in the extracts were determined within 25 min.
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