Abstract
1. 1. This paper confirms the usefulness of electrochemical detector (ED) for liquid chromatography in determining picogram amounts of catecholamines in mouse and rat brain. 2. 2. The extraction procedure was based on modifications of Kissinger's method and of Anton and Sayre, in which catecholamines were adsorbed on aluminum oxide and eluted with perchloric acid. 3. 3. The detector operates down to the 0.2 – 0.4 ng range. Norepinephrine (NE) levels were 431 ± 18 ng/g in mouse brain and 426 ± 19 ng/g in the rat. Dopamine (DM) levels were 1088 ± 66 ng/g in mouse brain and 787 ± 33 ng/g in the rat. 4. 4. We have found significant changes in DM levels in rat whole brain depending on the method of sacrifice. Level of NE in the forebrain after electrolytic destruction of the locus coeruleus were significantly decreased (24.2 %) from that of control.
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