Abstract

A method is described for calculating local myoglobin concentration in hemoglobin-free perfused guinea-pig heart by measuring the decrease of tissuePo2 during stop of perfusion. The myoglobin concentration in ca. 10 nanogram of tissue varies between 0.106 and 0.676 g-% of the wet weight. The mean value of 0.373 g-% (standard deviation of ±0.138) agrees very well with the values obtained with chemical methods by other authors. The great variation of our values is interpreted as an inhomogeneous distribution of the local myoglobin concentration.

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