Abstract
A sensitive analytical procedure for the determination of residues of leucogentian violet (LGV) and gentian violet (GV) in catfish tissue is presented. Frozen (−20°C) catfish fillets were cut into chunks and then blended in a Waring blendor. A 10-g amount of catfish muscle tissue was homogenized and extracted with acetonitrile-buffer, partitioned against methylene chloride, and cleaned up on tandem neutral alumina and propylsulfonic acid cation-exchange solid-phase extraction cartridges. Samples of 100 μl (0.5 g equiv.) were chromatographed isocratically in 15 min using an acetonitrile-buffer mobile phase on a cyano phase column in-line with a post-column PbO 2 oxidation reactor. The PbO 2 post-column reactor efficiently oxidized the LGV to the chromatic GV permitting visible detection at 588 nm for both LGV and GV. Linearity was demonstrated with standards over the range 0.5–50 ng per injection. Recoveries of LGV and GV from catfish tissues fortified at 20, 10, and 1 ng/g were 83.1 ± 1.2, 78.4 ± 4.0, 84 ± 8 and 92.7 ± 1.8, 95.0 ± 2.2, 93 ± 2 (mean ± S.D., n = 4), respectively.
Published Version
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