Abstract
A method is presented for the detection of the nitrofuran antibacterial, furazolidone in animal feeds. Following solvent extraction of feed samples, the extracts were purified on an alumina column. The column eluents were separated by HPLC using a reversed phase column and detected either by UV monitoring at 365 nm, or by thermospray mass spectrometry, monitoring the ion at m/z 243. The LC-UV procedure was used to quantify furazolidone at medicated levels (200 mg/kg) and at contamination levels of 5 and 20 mg/kg. The limit of detection was 1 mg/kg. Overall mean recoveries from fortified samples were, 93.4, 98.2 and 98.0% at concentrations of 5, 20 and 200 mg/kg, respectively. The mass spectrometric procedure was used to analyse sample extracts at concentrations <5mg/kg. The overall recovery from feed samples fortified at 1 mg/kg was 93.8%. The assay is therefore capable of the determination of furazolidone in animal feeds at both high medicated concentrations and at low contamination levels.
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