Abstract
ϵ-N-1-(1-Deoxylactulosyl)- L-lysine was synthesized and used as a substrate to assay β-galactosidase activity. ϵ-N-1-(1-Deoxylactulosyl)- L-lysine and its degradation product ϵ-N-1-(1-deoxyfructosyl)- L-lysine were detected by high-voltage paper electrophoresis and ion-exchange high-performance liquid chromatography. The β-galactosidase activity in different parts of the intestinal tract of germ-free and control mice was determined and compared with a β-galactosidase activity which degrades lactose at pH 8.5 and 5.0 and which corresponded with bacterial and host enzymatic activities, respectively.
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More From: Journal of Chromatography B: Biomedical Sciences and Applications
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