Abstract
ABSTRACT Three methods to detect nitric oxide (NO()) are reported here. The first method was determining NO() in extracted plant tissue. NO() was trapped by spin trapping reagent containing diethyldithiocarbamate (DETC) and FeSO(4), extracted by ethyl acetate, and determined with an electron spin resonance (ESR) spectrometer. The second method was indirectly determining NO() in live wheat leaves. Seedlings were cultured in a medium containing FeSO(4), and the leaves were brushed by DETC. Then, the leaves were ground and the complex of (DETC)(2)-Fe(2+)-NO was extracted and determined with an ESR spectrometer. The third method was directly determining NO* in live wheat leaves. After treating plant materials as in the second method, part of the water in leaves was transpired, and the leaf disks were inserted directly into quartz tubes to determine NO() with an ESR spectrometer. The NO() scavenger 2-phenyl-4,4,5,5,-tetramethylimidazoline- 1-oxyl 3-oxide (PTIO) decreased NO() signal detected either by an indirect or a direct method. This result indicates that both methods could detect NO() in the live plant. Using the first methods, we detected NO() change in wheat infected by Puccinia striiformis race CY22-2 pathogen (incompatible interaction) at different inoculation times, and it was found that the NO() content dramatically increased at 24 h postinoculation, quickly decreased at 48 h, and increased again at 96 h.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.