Abstract
A novel method based on determination of the degree of depolarization of scattered light has been proposed as a novel method for detecting defects in rigid chain macromolecules. In calculations of the scattering intensity a “hybrid” chain is introduced. The latter contains fragments of continuous curvature that are separated by points of high flexibility (local defects). Light scattering data for DNA subjected to ultrasonic degradation are analyzed on the assumption that a Poisson type distribution of defects is involved. Linear density calculations carried out for DNA single filament ruptures have demonstrated the efficiency of the method.
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