Abstract
目的 评价聚合酶链反应技术(PCR)对角膜内皮炎的诊断价值,并以此解释病因,指导临床治疗.方法对临床诊断为角膜内皮炎的患者12例(12眼)及临床诊断为年龄相关性白内障的患者15例(15眼)分别用PCR方法进行房水中单纯疱疹病毒Ⅰ型(HSV-Ⅰ)DNA及带状疱疹病毒(VZV)DNA的检测,结果采用分类变量资料两样本率比较的四格表确切概率比较.结果共扩增角膜内皮炎患者12例(12眼),HSV-Ⅰ阳性者5例(5眼),阳性率为41.67%,共扩增对照组15例(15眼),HSV-Ⅰ阳性者0例,阳性率为0%,二者差异有显著性.结论PCR技术检测角膜内皮炎患者房水中的HSV-Ⅰ,可在分子水平上建立一种快速准确的诊断方法,为临床治疗提供可靠的依据。
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