Detection of fish gelatin with DNA biomarkers

Abstract

The utilization of gelatin for food and non-food processing is increasing, but there are many mislabeling errors of the origin of commercial gelatin raw materials. This mislabeling can harm communities related to religion or safety. Therefore, research on detection of fish gelatin with Polymerase Chain Reaction is very important as a source of halal gelatin. This study aimed to isolate DNA of fish gelatin and determine the proper primers to identify fish gelatin using the DNA-based method. DNA was amplified using universal fish F1 / R1 primers, Mini-barcode, cyt b Pangasianodon sp., fish collagen, cyt b bovine, and 12S rRNA-tRNA Val Sus sucrofa. The catfish meat and fish gelatin samples could be amplified at an annealing temperature 59°C with universal fish primers F1R1, while the mini barcode and cyt b Pangasianodon sp. primers were carried out at 54 °C. Ish collagen gene primer could not amplify DP (catfish meat) and GI (fish gelatin) samples. The sequence of the DP and GI DNA samples amplified with cyt b Pangasianodon sp. primers was identical to Pangasianodon sp.