Detection of Enterotoxin type B gene on Methicillin Resistant Staphylococcus aureus (MRSA) isolated from raw milk in East java, Indonesia

Abstract

Background and Aim: Methicillin resistant Staphylococcus aureus (MRSA) has an exponential rate of spread throughout the world and its occurrence rates have been detected in animal / food samples of animal origin. The methicillin resistance (MR) strain is a significant factor in the potential virulence of Staphylococcus aureus such as the presence of Stapylococcal enterotoxin B (SEB) which induces a super antigenic effect. This study aims to detect the presence of SEB gene in MRSA isolates that isolated from cow milk in East Java province. Materials and Methods: Raw cow's milk ingredients were inoculated in enrichment media and mannitol salt agar (MSA) which were then tested using oxacillin and cefoxitin disc diffusion combined with oxacillin screen agar (ORSA) test to detect MRSA strains. All MRSA strains were detected by the SEB gene by the polymerase chain reaction (PCR) method. Results: In this study confirmed 18 MRSA from 150 samples of cow's milk and detected 4 MRSA isolates having SEB gene with product size 478 bp. In Conclusion: Detection of MRSA carrying the SEB gene in milk can have an impact on the public sector / animal health (milk borne disease) and the economy on livestock. It is very necessary to further analyze the relationship between various virulence factors in MRSA isolates that have the potential to be transmitted through cow's milk in East Java Keywords: Methicillin resistant Staphylococcus aureus(MRSA), Stapylococcal enterotoxin B (SEB), food safety, milk borne disease