Abstract
Two procedures to detect and purify covalently closed circular plasmid DNA present inThermus strains from Icelandic hot springs are described in detail. Using these procedures, plasmid DNA was detected in IcelandicThermus strains B1 and B2, and 0.2–0.4 mg of pure plasmid DNA was isolated from a litre of the cultures (8.5–9.0 g wet weight). Strain B1 harboured one plasmid species, pCLK 1; strain B2 harboured two plasmid species, pCLK 2 and pCLK 3.
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