Abstract
A novel procedure is described for the detection and determination of N-Nitrosamino acids (NAAs) on activated silica gel thin-layer chromatographic plates. N-Nitrososarcosine, N-nitrosoproline, and N-nitroso-4-hydroxyproline could be detected as fluorophors at the 200-pmole level (20–30 ng) after being irradiated with ultraviolet light and sprayed with fluorescamine reagent. Spectrophotometric determination of the relative fluorescence of 0.4–40 nmoles of NAAs gave rise to similar calibration curves when plotted on a log—log scale. An application of this method to the detection o NAAs in uncooked bacon is described.
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