Abstract
Procedures are described for the acylation of 20–35μg of hydroxy steroids. The reactions are performed on thin-layer silica gel plates. Acetic anhydride-pyridine and benzoyl chloride-pyridine mixtures are used as acylating agents. Dihydroxy steroids yield two products while trihydroxy steroids yield three products. Separation and purification of the reaction products are carried out on the same plate. Several hydroxy steroids can be simultaneously acylated on one thin-layer plate, and the microsyntheses are completed in about 2 hr. The steroid ester is identified by (a) comparision with authentic sample using thin-layer chromatography, or (b) hydrolysis to give the parent hydroxy steroid, or (c) mass spectrometry.
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