Detecting Polymorphisms of Mutation (C327T) rs 417415931 of Gene Foxo3 and its Associations with Reproductive Performance of Awassi Sheep

Abstract

The experimental samples for the current study included 45 sheep of the local Awassi breed, whose ages ranged between (2-4) years. These ewes produced 54 newborns, and their ages ranged from birth to the sixth month, and for the period from 1/2/2022 to 25/6/2022. The experiment was conducted. For this study, the field side was carried out at the Al-Fayhaa cow farm and station of the Directorate of Agriculture of Babylon / Ministry of Agriculture. As for the laboratory side, laboratory analyzes were conducted in the laboratories of the Scientific Advancement Company specialized in molecular genetics for the period from 1/5/2022 to 6/20/2022. With the aim of isolating the substance GeneticsDNA and the effect of the genetic morphology of the 417415931 (C327T) rs mutation and the study of the relationship of this genetic morphology with the productive traits represented in daily milk production (DMY), milk components, growth traits for lambs, traits related to maternal body dimensions, live weight of mothers and type of birth. This mutation was found in the plot. Studied with a molecular size of 365 bp) from exon number 2 for the Foxo3 gene, through the DNA nucleotide sequencing technique. This mutation produced three genotypes (CC, CT, and TT), with distribution ratios of (2.28, 36.36, 61.36)% sequentially, and the variance between these ratios was highly significant (p≤0.01), and the allelic frequency was (0.20, 0.80) for each of the C and T alleles. Respectively. It was found that the effect of the genotypes of the Foxo3 gene was not significant in the studied traits, as the ratios were close between the genotypes produced by the wild, the hybrid, and the pure genotype. It can be concluded from the results of this study not to rely on this mutation formed by the Foxo3 gene, and to adopt it as a genetic marker to improve productive traits or genital.