Abstract
In the present work, we proposed a novel naked-eye and turn-on fluorescence sensor based on the 5,10,15,20-(4-sulphonatophenyl) porphyrin (TPPS4)-Hg2+ system with short assay time and high sensitivity to detect glutathione (GSH) in real samples. The TPPS4 was the first time to detect GSH because of its high fluorescence quantum yield. The initial fluorescence from TPPS4 was quenched by Hg2+ with an electron transfer process. However, when put a certain concentration of GSH into this system, the fluorescence sensor was turned to the “on” state, which was related to a competitive affinity of Hg2+ to GSH and the N atoms of porphyrin ring. Under the optimal conditions, the naked-eye detection limit was 0.43nM and the limit of detection (LOD) was arrived to 16 fM (S/N=3) for GSH. The linear regions are obtained by two ranges varying from 0 to 28.5μM and from 38.15 to 61.87μM. Furthermore, it was confirmed that the TPPS4-Hg2+ fluorescence probe has excellent selectivity, high sensitivity and quick fluorescence response for GSH.
Published Version
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