Abstract
One of the main visualization method in neuroscience is two-photon microscopy. However, scientists need to upgrade their microscopy system so regular because they are interested to get more specific data. Also this is due to the fact that this technique allows to obtain 3D images of tissues due to laser focus change, that is possible due to substantially greater penetration depth on the main wavelength into biological tissues. Self-developed microscopy system allow to modify the construction of microscope in not-complicated manner depending on specialized experimental models and scientific tasks. This work is small attempt to help researchers who are interested to build their own two-photon laser scanning microscope for using in neuroscience. The work was performed with financial support of the government represented by the Ministry of Education and Science of the Russian Federation, the unique identifier of the project is RFMEFI57814X0079, the agreement on granting a subsidy №14.578.21.0079 dated 28.11.2014.
Highlights
Application of modern techniques offer high resolution properties for living systems research is significant key for neurobiology progress. there are certain peculiarities arising from the specifics of experiments using this technique
Working with selected methods require both of hightechnology equipment availability and knowledge and skills to applying non-trivial mathematical algorithms for control two-photon microscopy system [1] and for wellformed data analysis [2], [3]
Two-photon microscopy offers several advantages compared with other forms of microscopy
Summary
Application of modern techniques offer high resolution properties for living systems research is significant key for neurobiology progress. there are certain peculiarities arising from the specifics of experiments using this technique. Application of modern techniques offer high resolution properties for living systems research is significant key for neurobiology progress. There are certain peculiarities arising from the specifics of experiments using this technique. For example, the analysis of the morphological cell data of hippocampal slices of the brain of rodents, other experiments in vivo depending on the design using the full-sphere of the virtual reality or, for example, in the form of a cylindrical hover platform. Working with selected methods require both of hightechnology equipment availability and knowledge and skills to applying non-trivial mathematical algorithms for control two-photon microscopy system [1] and for wellformed data analysis [2], [3]. Two-photon microscopy offers several advantages compared with other forms of microscopy. This article may be regarded as a quick reference to laboratory staff who are wishing to develop their own microscopy system for self-service and modernization of the system and in order to save the lab budget
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