Dense line in cisterna of rough-surfaced endoplasmic reticulum of hepatocytes of the rat perfused with saponin solution

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The effect of brief perfusion of saponin solution to the membrane of rough-surfaced endoplasmic reticulum(r-ER) was observed in hepatocytes at electron microscope level. The liver of rats was perfused with 1% saponin solution in 0.2m phosphate buffer (pH 7.0) for 3 min. then perfused again with 4% glutaraldehyde buffered with 0.1m sodiumcacodylate-HCl at pH 7.4. The most striking alteration of ultrastructure of hepatocytes was the dense intracisternal line of the r-ER. That dense line often had cross striation or “ladder structure”. Although its functional significance remains to be cleared, it should reflect the denature of membrane structure of r-ER as one of effects of saponin.