Voltammetric Determination of Dexamethsone in Serum, Urine and Drugs

Abstract

The present work involves a study of the polarographic behaviour of pure Dexamethasone by direct method in aqueous solution , which shows a well defined reduction peaks at a potential (-1.08) V. in phosphate buffer (pH=7) and (-0.84) V. in phosphate buffer (pH=3). The calibration of Dexamethasone was constructed in phosphate buffer (pH=3and7) for the range of concentration (2.4910-6M)-(1.5410-5M)in phosphate buffer (pH=7) with a correlation coefficient (0.9949), and with a range of concentration (6.310-7M)-(1.5410-5M) in phosphate buffer (pH=3) with a correlation coefficient (0.9957). The calibration curve of Dexamethasone in presence at human blood serum in the range of concentration (3.1110-6M)-(9.2810-6M) in phosphate buffer (pH=7) with a correlation coefficient (0.9892), and range of concentration (6.210-7M)-(6.2110-6M) in phosphate buffer (pH=3) with a correlation coefficient (0.9873). The calibration curve of Dexamethasone in presence of urine in the range of concentration (1.8610-6M)-(1.5310-5M) in phosphate buffer (pH=7) with a correlation coefficient (0.9813), and with a range of concentration (6.210-7M)-(1.2310-5M) in phosphate buffer (pH=3) with a correlation coefficient (0.9974). The method was successfully applied to the determination of Dexamethasone in tablets and syrup in aqueous solution.