Abstract
The degradation kinetics of growth hormone-releasing hexapeptide (GHRP-6) as a function of pH and temperature as well as buffer species and ionic strength has been studied by stability-indicating reversed-phase gradient high-performance liquid chromatography. Degradation chromatograms were confirmed in different pH solutions and the observed degradation reaction rates approximately followed first-order kinetics with respect to GHRP-6. The influence of the various buffer species (acetate, citrate, phosphate and borate) was shown to be different and the maximum stability of GHRP-6 was revealed to be in acetate buffer of pH 5.5–6.0. Degradation of GHRP-6 was greater in citrate-containing buffers than in acetate-containing ones. Furthermore, in the citrate-containing buffers, the higher buffer concentration caused greater degradation than the lower ones, but the concentration effect was negligible in acetate-containing buffers. Aqueous solution of GHRP-6 buffered with acetate (0.01 M, pH 5.5) showed a predicted t90% of 4.73 years at 20°C.
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