Abstract
Ribosomes from T4-infected Escherichia coli when isolated with their initiation factors still attached have negligible activity for the translation of f2 RNA but retain activity for the translation of late T4 RNA. When low concentrations of f2 RNA are used with T4 ribosomes which contain factors, formylmethionyl-tRNA does not bind in response to f2 RNA. Yet the latter ribosomes show substantial activity in binding formylmethionyl-tRNA in response to high concentrations of f2 RNA. Failure of the aberrant initiation complexes to function in translation does not appear to be related to the amount of initiator tRNA bound.
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