Abstract
In this paper, laccase enzyme was immobilized onto zinc ferrite nanoparticle and the characteristics of enzyme immobilized nanoparticle (EIN) were evaluated by Fourier transform infrared (FTIR) and scanning electronic microscope (SEM). Enzymatic decolorization of dyes using EIN from single and binary systems was studied. Direct red 31 (DR31) and Acid blue 92 (AB92) were used as model dyes. The effects of several parameters such as EIN dosage, pH, and dye concentration on decolorization of dyes from single and binary systems were evaluated. The optimized amount of EIN, reaction time, and pH for decolorization of dyes from single and binary systems were 0.2 g (for DR31) and 0.3 g (for AB92), 40 min, and 3 in single systems and 0.2 g (for DR31 and AB92), 40 min, and 3 in binary systems, respectively. Dye decolorization kinetics followed Michaelis-Menten model. The results showed that enzymatic process using EIN was effective method to decolorize dyes from single and binary systems.
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