Abstract
In this paper, immobilized laccase enzyme on nano zinc ferrite was used in order to decolorize disperse dyes from single and binary systems. In this case, disperse dyes such as Disperse red 60 (DR60), Disperse blue 56 (DB56) and Disperse yellow 54 (DY54) were selected as model dyes. Several parameters such as enzyme concentration, pH and dye concentration and their effect on decolorization of dyes from single and binary systems were studied. According to the experimental results, the optimized immobilized laccase enzyme concentration, reaction time and pH for decolorization of DR60, DB56 and DY54 from single and binary systems were 500 mg/L (for DR60 and DY54) and 400 mg/L (for DB56), 20 min and 3, respectively. Moreover, Dye decolorization kinetics followed Michaelis-Menten Model. Finally, the results showed that enzymatic process using immobilized laccase enzyme on nano zinc ferrite was effective method to decolorize disperse dyes from single and binary systems.
Highlights
IntroductionDye removal of industrial effluent has been a major concern in wastewater treatment, especially for textile and dyestuff plants
In this paper, immobilized laccase enzyme on nano zinc ferrite was used in order to decolorize disperse dyes from single and binary systems
The results showed that enzymatic process using immobilized laccase enzyme on nano zinc ferrite was effective method to decolorize disperse dyes from single and binary systems
Summary
Dye removal of industrial effluent has been a major concern in wastewater treatment, especially for textile and dyestuff plants. Laccases from white rot fungi are potential industrial enzymes in various applications such as pulp delignification, wood fiber modification, chemical or medicinal synthesis and waste water treatment [5]. Different factors such as highly polluted waste water and mediator-by products have significant effect on decreasing of stability and catalytic ability of free enzyme. Laccase enzymes from white rot fungi with high enzymatic activity have found many applications in various industries, such as pulp delignification, wood fiber modification, chemical or medicinal syntheses, and wastewater treatment [7]. The effect of several parameters such as enzyme concentration, pH and dye concentration on decolorization of dyes from single and tertiary systems was evaluated
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