Immobilization of laccase enzyme onto titania nanoparticle and decolorization of dyes from single and binary systems

Abstract

Laccase enzyme was immobilized on titania nanoparticles and the resulting nanoparticles (ILTN) were characterized with various techniques: FTIR and SEM. ILTN was used to degrade three anionic dyes (Direct Red 31 (DR31), Acid Blue 92 (AB92), and Direct Green 6 (DG6)) from aqueous solution, individually and in binary systems. The effect of various parameters (e.g., ILTN dosage, pH, and dye concentration) on decolorization was evaluated and the optimized conditions were determined. The amount of ILTN, reaction time, and pH for decolorization of DR31, AB92 and DG6 was 0.1 g (for DR31 and DG6) and 0.3 g (for AB92), 20 min, and pH 3 in single systems and 0.3 g, 20 min and pH 3 in binary systems, respectively. Decolorization followed Michaelis-Menten kinetics.