Decolorization of cationic dyes under alkaline conditions by Iodidimonas sp. Q-1 multicopper oxidase

Abstract

Previously, we found that a multicopper oxidase (IOX) produced by Iodidimonas sp. Q-1, an iodide (I-)-oxidizing marine bacterium, exhibited significant decolorization activity toward various anionic dyes. In this study, the potential capacity of IOX for decolorization of cationic dyes such as malachite green (MG), crystal violet (CV), and methylene blue (MB) was determined. Decolorization of the dyes by IOX exhibited significant pH dependence, and effective decolorization was observed under alkaline conditions. At an optimum pH of 9.5, IOX decolorized more than 90% of MG, CV, and MB within 30min, 2h, and 6h, respectively. The addition of iodide was indispensable for decolorization, suggesting that this halide ion serves as a redox mediator. Decolorization products of MG showed less toxicity than MG against Escherichia coli cells. These results suggest that this IOX-iodide system can be used for the decolorization and detoxification of cationic dyes under alkaline conditions.