Abstract
THE presence of synapses in long term organ cultures of mammalian and avian nervous tissue has been demonstrated previously by light and electron microscopic and neurophysio-logical studies1–5. A close correlation of the appearance of synapses and the onset of bioelectric activity has also been reported in cultures of foetal rat spinal cord6, 7. Here we report that silver and electron microscopic studies of cultures of chick neural tube reveal morphological evidence of de novo formation of synapses on cells differentiated from neuroepithelial cells, the precursors of the neuronal elements.
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