Abstract
We report a draft genome assembly of Streptomyces sp. strain Mg1, a competitive soil isolate with multiple secondary metabolite gene clusters.
Highlights
Whole-genome shotgun sequencing of the Mg1 strain was carried out using PacBio SMRT sequencing technology [2]
For assembly of the Mg1 genome, we applied the recently described hierarchical genome assembly process (HGAP) to eight SMRT cells of sequencing data generated from an 8- to 10-kb insert library [3]
High coverage of short reads from 454 GSFLX titanium and Illumina HiSeq 100-bp paired-end data did not resolve the problems with the previous assembly
Summary
Whole-genome shotgun sequencing of the Mg1 strain was carried out using PacBio SMRT sequencing technology [2]. For assembly of the Mg1 genome, we applied the recently described hierarchical genome assembly process (HGAP) to eight SMRT cells of sequencing data generated from an 8- to 10-kb insert library [3]. Greater than 90% of the predicted genome size is contained within one large 7.8-Mb contig. The remaining sequence is divided into smaller contigs 50 to 500 kb in length.
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