Abstract
<div>Abstract<p>Recently, long noncoding RNAs (lncRNA) have been reported as tumor suppressors or oncogenes in colorectal cancer. This study aims to discover functional role of a novel lncRNA in colorectal cancer tumorigenesis. Expression profile of fibronectin type III domain containing 1 antisense RNA 1 (<i>ELFN1-AS1</i>) in colorectal cancer samples was displayed on TCGA database. Expression level of <i>ELFN1-AS1</i> was tested in colorectal cancer tissues and cell lines via qRT-PCR. Functional role of <i>ELFN1-AS1</i> was assessed by loss-of-function assays. Mechanism experiments, such as chromatin immunoprecipitation (ChIP) assay and luciferase reporter assay, were done to analyze the molecular mechanism of <i>ELFN1-AS1</i> in colorectal cancer. <i>ELFN1-AS1</i> knockdown inhibited colorectal cancer tumor growth through restricting cell proliferation and facilitating cell apoptosis. <i>ELFN1-AS1</i> was transcriptionally activated by MYC. Moreover, <i>ELFN1-AS1</i> led to transcriptional silencing of tropomyosin 1 (TPM1) via recruiting enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) and forkhead box P1 (FOXP1). Collectively, MYC-upregulated <i>ELFN1-AS1</i> recruited EZH2 and FOXP1 to restrain TPM1 expression, thereby promoting colorectal cancer tumor growth.</p>Implications:<p>This study revealed a novel molecular pathway in colorectal cancer progression, which may provide new method for early diagnosis and treatment of colorectal cancer.</p></div>
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