Cytoskeletal control of alanine transport across the rat and turtle small intestine

Abstract

1. 1. Procaine inhibited significantly ( P < 0.01) alanine accumulation in the rat intestinal strips in a concentration-dependent pattern, whereas it showed no effect on alanine uptake by the turtle intestinal cells. 2. 2. Colchicine and Vinca alkaloids at 5 × 10 −4 and 1.5 × 10 −6 M respectively caused a significant inhibition ( P < 0.01) of intracellular alanine concentration in the rat with no effect noticed in the turtle. 3. 3. Unidirectional influx of alanine across the brush border membrane of the rat was significantly ( P < 0.01) reduced in the presence of procaine, colchicine and vincristine in the preincubation medium. The same drugs did not show any effect on alanine influx into the turtle small intestine. 4. 4. Electron microscopy showed major structural alterations in the cytoskeletal organization of the turtle intestine in response to procaine, colchicine or vincristine treatment. 5. 5. It is proposed that microtubular system may participate in the overall transport mechanism of alanine across the small intestine.