Abstract
The cytoplasmic male sterile (CMS) wheat (Triticum aestivum L.) line KTP116A developed at Northwest AF most of those became abnormal pollen grains and shell structures without protoplast, confirming that the conversion from sterility to fertility in the CMS line was accompanied by changes in morphology and cytology. A BC1 population of 198 plants from a cross of male-sterile KTP116A and male fertile F1 TP116B/WM5-5 was developed to study the genetic control of thermo-sensitive sterility. Chi squared tests on data from back-crossed populations revealed that two recessive genes, designated rfv and rfv 2 , were responsible for sterility of line KTP116A. Sixteen of 712 SSR markers were polymorphic between the parents and bulks. Four SSR markers, viz. Xgwm11, Xgwm18, Xgwm413 and Xbarc137, were linked to thermo-sensitive gene rfv on chromosome 1BS of T. spelta, and another four markers were linked to rfv 2 located on chromosome 2A. This thermo-sensitive male sterile line can be used for production of experimental hybrids in order to test levels of heterosis.
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