Abstract
Super-resolved cryogenic correlative light and electron microscopy (srCryoCLEM) is an exciting new method that can determine the positions of specifically labelled biomolecules of interest in the context of cryogenic electron tomography (CET) reconstructions. These srCryoCLEM experiments are conducted by sequentially performing cryogenic superresolution fluorescence followed by CET. Biological samples for srCryoCLEM are rapidly frozen to preserve the sample in its native hydrated state. Further, this rapid freezing produces amorphous (vitreous) ice as opposed to crystalline ice that would obscure the transmitted electron beam.
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