Curcumin Coated Orthosiphon Stamineus Leaf Extract Based Selenium Nanoparticle for Potential Tissue Engineering Applications

Abstract

Curcumin is a type of polyphenol phytochemical that is bright yellow in colour and produced by the plant Curcuma longa. Despite its pharmacological properties, curcumin has low bioavailability, poor solubility, and undergoes rapid degradation. Nanoparticles (NPs) are used as a nanocarrier for drug delivery, to improve the stability and pharmacokinetics of the drug. Therefore, by coating curcumin over selenium NPs (SeNPs), the bioactivity, bioavailability, stability, and may increase the solubility of curcumin of SeNPs. This study aimed to synthesize the SeNPs from Orthosiphon stamineus leaf extract and coat it with curcumin and to characterize it and check its biocompatibility. Biosynthesis of SeNPs was carried out using plant extract of Evolvulus alsinoides and characterized using UV spectrophotometer, FT-IR, and SEM. Annexin V PI apoptotic and Hemolytic assay were used for checking biocompatibility. The UV-Vis spectrum gave a strong peak at 265 and 423 nm at various time intervals, indicating the SeNPs formation. Similarly, FT-IR has strong absorption bands at 3279, 1284, 1072, 1028, and cm−1 with wavelengths ranging from 4000-500 cm-1. SEM analysis of biosynthesized SeNPs showed a spherical shape. Our results suggest that curcumin-coated SeNPs possess greater biocompatibility towards PBMCs which was evaluated by Annexin V - PI assay and erythrocytes by hemolytic studies. Curcumin-coated Selenium nanoparticles were successfully synthesized by the biological method using leaf extract of Orthosiphon stamineus and reported as biocompatible using Flow cytometry. But a more detailed study should be done for implementing it in tissue engineering. Keywords: Selenium nanoparticle, Curcumin, Orthosiphon stamineus, Tissue Engineering.