Green Synthesis of Selenium Nanoparticles by Cyanobacterium Spirulina platensis (abdf2224): Cultivation Condition Quality Controls.

Shohreh Alipour,Ali Dehshahri,Sara Kalari,Zahra Sabahi,Mohammad Hossein Morowvat,Ashok Nadda

doi:10.1155/2021/6635297

Shohreh Alipour, Ali Dehshahri + Show 4 more

Open Access

https://doi.org/10.1155/2021/6635297

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Abstract

Selenium nanoparticles (SeNPs) are well-known bioactive compounds. Various chemical and biological methods have been applied to SeNP synthesis. Spirulina platensis is a widely used blue-green microalgae in various industries. In this study, the biosynthesis of SeNPs using sodium selenite and Spirulina platens has been developed. The SeNP synthesis was performed at different cultivation condition including pH and illumination schedule variation. The SeNPs were characterized by FT-IR, XRD, size, and zeta potential measurements, and the antioxidant activities of selected SeNPs were evaluated by DPPH and FRAP assays. FT-IR analysis showed the production of SeNPs. The 12 h dark/12 h light cycles and continuous light exposure at pH 5 led to the production of stable SeNPs with sizes of 145 ± 6 and 171 ± 13 nm, respectively. Antioxidant activity of selected SeNPs was higher than sodium selenite. It seems that green synthesis is a safe method to produce SeNPs as well as a convenient method to scale-up this production.

Highlights

Great attention has been directed to selenium as one of the significant dietary supplements
The S. platensis growth pattern exhibited a sigmoidal growth curve with a 2-day lag phase which led to a log phase that was started on the 3rd day after culturing and reached to a maximum on the 5th day
The results of our study demonstrated that the cyanobacterial samples enter the dead phase after 6 days and could not be suggested for the synthesis of nanoparticles

Summary

Introduction

Great attention has been directed to selenium as one of the significant dietary supplements. The importance of selenium in human diet comes from the fact that this element is present in the structure of proteins including glutathione peroxidase, thioredoxin reductase, and deiodinases. These enzymes act as scavengers of reactive oxygen spices (ROS) with antioxidant activity [1, 2]. Some of the selenium supplements, the inorganic forms, have shown toxicity in higher nutritional doses. SeNPs were developed to reduce its toxicity and improve its biological activities [4]. Considerable anticancer and antioxidant activities have been reported for selenium nanoparticles.

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Conclusion