Curative effects of the aqueous extract of Tithonia diversifolia (Hemsl.) A. gray (Asteraceae) against ethanol induced-hepatotoxicity in rats.

Abstract

Tithonia diversifolia (Asteraceae) is used in Cameroonian traditional medicine for the treatment of several diseases amongst which are hepatic disorders. Anti-inflammatory, analgesic and anti-diabetic properties have been reported but, there is no scientific information on its hepato-protective effects. The aim of this study was to evaluate the curative effects of the Tithonia diversifolia (T.diversifolia) leaves aqueous extract on ethanol induced-hepatotoxicity in rats. Ethanol 40° (4g/kg) was administered daily by intragastric gavage for 21days, and then the extract was administered concomitantly with ethanol for two more weeks. Some biochemical serum and tissue parameters were evaluated. Histopathologic analysis of the liver was carried out. The ingestion of ethanol induced a significant reduction of body weight and a significant increase in some markers of hepatic function (Alanine Amino-transferase, Aspartate Amino-transferase, alkaline phosphatase, gamma glutamyl-transferase, total bilirubin and albumin). These alterations were accompanied by a significant increase in the levels of serum triglycerides (p<0.001). Intoxicated animals were also characterized by a significant decrease of reduced glutathione and nitrites concentrations, catalase and superoxide dismutase activities as well as an increase of malondialdehyde levels. The histopathological examination showed vascular congestion, disorganized parenchyma, liver inflammation and dilation of sinusoid. The extract at the doses of 60 and 120mg/kg reversed ethanol-induced adverse effects. Our study found that, the aqueous extract of T.diversifolia leaves has hepato-protective activity against ethanol-induced liver damages due partly to its antioxidant effect. This result justifies its empirical use for the treatment of liver problems.