Abstract
The present study was carried out to test an in vivo crown gall tumor disc bioassay using potato discs of the aqueous and ethanolic extracts of Annona reticulata with Allium sativum, Allium fistolisum, Brassica oleraceae, and then correlate the cytotoxicity results with the known pharmacological activities of the plants. Cytotoxicity was evaluated according to the crown gall tumor disc bioassay, a highly specific, quantitative method that requires only a short period of time to return results of crown gall tumor formation. The results shows the cumulative activity of the extracts of Annona reticulate extract with Allium sativum, Allium fistolisum and Brassica oleraceae extracts and shown they were potent against the bioassay as compared to their being used alone with combined extracts. The results indicated that their bioactive components have considerable pharmacological effects. Thus, the results supported the uses of these plant species in traditional medicine.
Highlights
Crown gall is a neoplastic disease of plants that occurs in more than 60 families of dicotyledons and many gymnosperms
A definite correlation exists between tumor formation of these samples, and their ability to inhibit crown gall tumor formation on the potato discs
The amount of inhibition obtained with the active samples was consistent whether or not these extracts were added to the potato discs
Summary
Crown gall is a neoplastic disease of plants that occurs in more than 60 families of dicotyledons and many gymnosperms. The disease is characterized by the transformation of normal plant cells into autonomous tumor cells in a short period of time. The tumor possesses the capacity for autonomous growth independent of the normal control mechanism of the host [1]. The relevance of the crown gall tumor system to the general cancer problem has been thoroughly reviewed [3,4]. The use of highly specific, quantitative bioassays that require only a short period of time to obtain results are available for studying crown gall tumor formation [5,6]. Using the potato disc bioassay we examined extracts and purified compounds of plant origin, some of which had shown antitumor activity in animals, for their effect on the initiation of crown gall tumors
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