Abstract

The present study was conducted to test for in vivo Brine Shrimp Lethality Assay (BSLA) of the Aqueous and ethanolic extracts of Annona reticulata with Allium fistolisum and Brassica oleraceaeand correlate cytotoxicity results with known pharmacological activities of the plants. Cytotoxicity was evaluated in terms of LC50 (lethality concentration). Ten nauplii were added into three replicates of each concentration of the plant extract. After 24 h the surviving brine shrimp larvae were counted and LC50 was assessed. Results showed that the extracts of Annona reticulata with Allium fistolisumand Brassica oleraceaewere potent against the brine shrimp when compared alone with combined extracts. It indicated that bioactive components are present in these plants that could be accounted for its pharmacological effects. Thus, the results support the uses of these plant species in traditional medicine.

Highlights

  • The crushed leaves of A. reticulata are used as poultice on boils, ulcers and abscesses and leaf decoction is used as vermifuge

  • The brine shrimp lethality assay (BSLA) is a simple and inexpensive bioassay used for testing the efficacy of phytochemical present in the plant extracts

  • The result on the lethality of Alcoholic and aqueous extract of Allium fistolisum on brine shrimps is with LC50 values (Tables 1 and 2) are 13.433mg/mL and 1846.550 mg/mL

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Summary

Introduction

The crushed leaves of A. reticulata are used as poultice on boils, ulcers and abscesses and leaf decoction is used as vermifuge. It is erect, with a rounded or spreading crown and trunk 10 to 14 in (25–35 cm) thick. Height ranges from 15 to 35 ft (4.5–10 m). The ill-smelling leaves are deciduous, alternate, oblong or narrow-lanceolate, 4 to 8 in (10–20 cm) long, 3/4 to 2 in (2–5 cm) wide, with conspicuous veins. Taking all the above concerns into account, we conducted this study to find out more about A

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