Abstract
Crystalloids have been observed by light microscopy in Sertoli cells of both experimentally induced and spontaneous azoospermic testes of the swine and in Sertoli cells of normal human testes. However crystalloids have not been found in normal swine testes with the light microscope. Swine crystalloids are distinguished from human crystalloids in that the diameter of the filaments is 5 nm instead of 10-15 nm. This paper confirms this distinction by showing that the filaments of swine crystalloids bind heavy meromyosin (HMM) but those of human crystalloids do not.Human testes were removed from four patients with prostate cancer, whereas normal and cryptorchid swine testes were acquired from a slaughterhouse. HMM extracted from rabbit skeletal muscle was dissolved in 10 mM Tris buffer (pH 7. 4) containing 0. 1 M KCI and 0. 001 M MgCl2 at a final concentration of 7 mg/ml. Pieces of testicular tissue were exposed to HMM at 4°C for 24 hours after glycerination or saponin treatment.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
