Cross-species Amplification of Scallop Microsatellites in the Family Pectinidae

Min Hui

doi:10.11648/j.eeb.20190404.12

Abstract

In the present study, 103 microsatellite markers were selected for cross-species amplification in four marine economic scallop species (Argopecten irradians, Chlamys farreri, Chlamys nobilis, and Mizuhopecten yessoensis) belonging to the family Pectinidae. As a result, in the 72 markers originated from C. farreri, 12 showed amplification products in C. nobilis, 12 in A. irradians and 11 in M. yessoensis. In the 12 markers developed from M. yessoensis, four markers could be amplified successfully in A. irradians, three in C. farreri, and one in C. nobilis. In the nine markers isolated from C. nobilis, two markers could be cross-amplified in A. irradians and one in M. yessoensis, but no marker was likely to be useful in C. farreri. In the nine markers of A. irradians, one marker was potentially workable for C. farreri, one in M. yessoensis, but none in C. nobilis. When the microsatellites were cross-species amplified, most of the PCR products showed low yield and ambiguous bands, while the numbers of alleles also decreased. Finally, three markers (CFMS016, CFCD131 and CFE04) were revealed to be successfully transferred among the four species providing candidate markers for ecological study of scallops, while most of the other markers were unique for one species with poor cross-species amplification, which might be useful for species identification.

Highlights

Scallops Argopecten irradians, Chlamys farreri, Chlamys nobilis and Mizuhopecten yessoensis are species belonging to the different genera of family Pectinidae, which are all important economic shellfish in China
103 microsatellite DNA marker’s transferability was further evaluated in the four scallop species in order to develop potential markers for related species, especially to find markers that can be amplified in all four species to facilitate the ecology investigation and even to use them to evaluate the phylogenetic relationships of scallops
Six individuals of each scallop species, A. irradians, C. farreri, C. nobilis and M. yessoensis were selected, which were popularly cultured in the seacoast of China

Summary

Introduction

Scallops Argopecten irradians, Chlamys farreri, Chlamys nobilis and Mizuhopecten yessoensis are species belonging to the different genera of family Pectinidae, which are all important economic shellfish in China. Their living conditions are heterogenous, which determines their distribution areas. Transferabilities of microsatellite markers in related species have been studies in many shellfish, such as in Crassostrea gigas [6] and Haliotis corrugata [7]. 103 microsatellite DNA marker’s transferability was further evaluated in the four scallop species in order to develop potential markers for related species, especially to find markers that can be amplified in all four species to facilitate the ecology investigation and even to use them to evaluate the phylogenetic relationships of scallops

Methods

Results

Discussion

Conclusion