CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum

Jingjing Xu,Subba Reddy Palli,Amit Roy

doi:10.1038/s41598-018-19667-6

Jingjing Xu, Subba Reddy Palli + Show 1 more

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https://doi.org/10.1038/s41598-018-19667-6

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Abstract

Juvenile hormones (JH) and ecdysteroids regulate many biological and metabolic processes. CREB-binding protein (CBP) is a transcriptional co-regulator with histone acetyltransferase (HAT) activity. Therefore, CBP is involved in activation of many transcription factors that regulate expression of genes associated with postembryonic development in insects. However, the function of CBP in JH action in insects is not well understood. Hence, we studied the role of CBP in JH action in the red flour beetle, Tribolium castaneum and the Tribolium cell line. CBP knockdown caused a decrease in JH induction of genes, Kr-h1, 4EBP and G13402 in T. castaneum larvae, adults and TcA cells whereas, Trichostatin A [TSA, a histone deacetylase (HDAC) inhibitor] induced the expression of these JH-response genes. Western blot analysis with specific antibodies revealed the requirement of CBP for the acetylation of H3K18 and H3K27 in both T. castaneum and TcA cells. Chromatin immunoprecipitation (Chip) assays showed the importance of CBP-mediated acetylation of H3K27 for JH induction of Kr-h1, 4EBP, and G13402 in TcA cells. These data suggest that CBP plays an important role in JH action in the model insect, T.castaneum.

Highlights

Juvenile hormones (JH) and ecdysteroids regulate many biological and metabolic processes
The Tribolium castaneum cell line (TcA) cells exposed to 10 μM JH III and showed an increase in krüppel homolog 1 (Kr-h1), 4E-binding protein (4EBP) and G13402 mRNA levels by 77.3, 3.2 and 3.2fold respectively, when compared to their levels in cells treated with DMSO (Fig. 1)
We investigated the role of this multifunctional protein in JH action

Summary

Introduction

Juvenile hormones (JH) and ecdysteroids regulate many biological and metabolic processes. Chromatin immunoprecipitation (Chip) assays showed the importance of CBP-mediated acetylation of H3K27 for JH induction of Kr-h1, 4EBP, and G13402 in TcA cells These data suggest that CBP plays an important role in JH action in the model insect, T.castaneum. Current studies on JH signaling pathway revealed mechanisms of JH action as well as its cross-talk with 20-hydroxyecdysone (20E), insulin signaling and WNT pathways[3,4,5,6,7,8,9,10,11] Most of these studies focused on understanding how JH regulates gene expression in the model insects including the fruit fly, mosquito, red flour beetle, cockroach and silk moth. In Galleria mellonella, histone acetylation has been shown to enhance transcription during insect metamorphosis, wounding and infection[27]

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