Abstract
BackgroundJuvenile hormones (JH) and ecdysteroids control postembryonic development in insects. They serve as valuable targets for pest management. Hence, understanding the molecular mechanisms of their action is of crucial importance. CREB-binding protein (CBP) is a universal transcriptional co-regulator. It controls the expression of several genes including those from hormone signaling pathways through co-activation of many transcription factors. However, the role of CBP during postembryonic development in insects is not well understood. Therefore, we have studied the role of CBP in postembryonic development in Tribolium, a model coleopteran insect.ResultsCBP is ubiquitously expressed in the red flour beetle, Tribolium castaneum. RNA interference (RNAi) mediated knockdown of CBP resulted in a decrease in JH induction of Kr-h1 gene expression in Tribolium larvae and led to a block in their development. Moreover, the injection of CBP double-stranded RNA (dsRNA) showed lethal phenotypes within 8 days of injection. RNA-seq and subsequent differential gene expression analysis identified CBP target genes in Tribolium. Knockdown of CBP caused a decrease in the expression of 1306 genes coding for transcription factors and other proteins associated with growth and development. Depletion of CBP impaired the expression of several JH response genes (e.g., Kr-h1, Hairy, early trypsin) and ecdysone response genes (EcR, E74, E75, and broad complex). Further, GO enrichment analyses of the downregulated genes showed enrichment in different functions including developmental processes, pigmentation, anatomical structure development, regulation of biological and cellular processes, etc.ConclusionThese data suggest diverse but crucial roles for CBP during postembryonic development in the coleopteran model insect, Tribolium. It can serve as a target for RNAi mediated pest management of this stored product pest.
Highlights
Juvenile hormones (JH) and ecdysteroids control postembryonic development in insects
Prior to metamorphosis, consists of a number of successive instars that are punctuated by molts that are regulated by major hormones, ecdysteroids and Juvenile Hormones (JH). 20- hydroxyecdysone (20E) is the major functional ecdysteroid that regulates molting and metamorphosis [1]. 20E binds to ecdysteroid receptor (EcR) and Ultraspiracle (USP) in target tissues leading to an
CREB-binding protein (CBP) expression in Tribolium larvae To study the expression of CBP gene during larval development, whole larvae and pupae were collected for RNA extraction at 24 h intervals during penultimate and final instar larval and pupal stages. quantitative reverse-transcriptase PCR (qRT-PCR) was performed with gene specific primers (Additional file 1: Table S1)
Summary
Juvenile hormones (JH) and ecdysteroids control postembryonic development in insects. They serve as valuable targets for pest management. CREB-binding protein (CBP) is a universal transcriptional co-regulator It controls the expression of several genes including those from hormone signaling pathways through co-activation of many transcription factors. We have studied the role of CBP in postembryonic development in Tribolium, a model coleopteran insect. 20E binds to ecdysteroid receptor (EcR) and Ultraspiracle (USP) in target tissues leading to an Insect juvenile hormone (JH) regulates many aspects of insect life including reproduction and development. Roy et al BMC Genomics (2017) 18:996 regulated by JH have been identified in these insects, and one gene consistently identified as an important player in JH action is krüppel homolog 1 (Kr-h1) [16,17,18,19,20,21]. The expression of Kr-h1 is directly induced by JH through Met, steroid receptor co-activator (SRC) and juvenile hormone response elements (JHRE) present in the promoter region [18, 24, 25]
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