Abstract
BackgroundEpigenetic modifications including DNA methylation and post-translational modifications of histones are known to regulate gene expression. Antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs) mediate transcriptional reprogramming during insect development as shown in Drosophila melanogaster and other insects. Juvenile hormones (JH) play vital roles in the regulation of growth, development, metamorphosis, reproduction and other physiological processes. However, our current understanding of epigenetic regulation of JH action is still limited. Hence, we studied the role of CREB binding protein (CBP, contains HAT domain) and Trichostatin A (TSA, HDAC inhibitor) on JH action.ResultsExposure of Tribolium castaneum cells (TcA cells) to JH or TSA caused an increase in expression of Kr-h1 (a known JH-response gene) and 31 or 698 other genes respectively. Knockdown of the gene coding for CBP caused a decrease in the expression of 456 genes including Kr-h1. Interestingly, the expression of several genes coding for transcription factors, nuclear receptors, P450 and fatty acid synthase family members that are known to mediate JH action were affected by CBP knockdown or TSA treatment.ConclusionsThese data suggest that acetylation and deacetylation mediated by HATs and HDACs play an important role in JH action.
Highlights
Immature juvenile forms of animals transform to become reproductive adults during their life cycle
These data suggest that acetylation and deacetylation mediated by histone acetyltransferases (HATs) and histone deacetylases (HDACs) play an important role in Juvenile hormones (JH) action
We studied the contribution of CBP (HAT, acetylation) and Trichostatin A (TSA) (HDAC inhibitor) to JH action
Summary
Immature juvenile forms of animals transform to become reproductive adults during their life cycle. Our previous work in T. castaneum has documented the dopamine D2-like receptor [G-protein-coupled-receptor (GPCR) family member] playing critical roles in regulating JH-mediated Vg uptake [11]. Cai et al, [12] reported the involvement of GPCRs during the 20E signaling suggesting that membrane receptors may be involved in this hormone signaling. It has been proposed that JH could regulate Ca2+ homeostasis within the cell during metamorphosis by interacting with membrane receptors or ion channels. Antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs) mediate transcriptional reprogramming during insect development as shown in Drosophila melanogaster and other insects. Juvenile hormones (JH) play vital roles in the regulation of growth, development, metamorphosis, reproduction and other physiological processes. We studied the role of CREB binding protein (CBP, contains HAT domain) and Trichostatin A (TSA, HDAC inhibitor) on JH action
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
