Cox multivariate analysis of T-bet effects on the survival of gastric carcinoma

Abstract

Objective To anabze the expression of T-bet in gastric carcinoma tissues and the impact on the survival time of patients.Methods The number of T-bet positive lymphocyte in the surgical specimens of gastric cancer tissues was detected by using immunohistochemistry in 152 patients.The time of disease-free survival and overall survival were observed.Data was analyzed by using SPSS 13.0.Results The T-bet expression level was significantly correlated with tumor size (t =2.35,P ＜ 0.05),invasion depth (t =2.50,P ＜ 0.05) and UICC stage (t =3.40,P ＜ 0.05).The median time of disease-free survival and overall survival were 28 months and 37 months.Kaplan-Meier survival analysis demonstrated that the disease free survival as well as the overall survival for the group with the higher density of T-bet positive lymphocyte was significantly better than the group with lower T-bet positive lymphocyte (X2 =3.91,P ＜0.05 and x2 =4.00,P ＜ 0.05,respectively).COX multivariate analysis showed that,after regulating age,sex,tumor location,depth of invasion,tumor size and UICC stage,the high infiltration group of T-bet positive lymphocyte had the trend of recurrence [HR =0.56,95％ confidence interval (95％ CI) =0.32-0.98,P ＜ 0.05] and death (HR =0.55,95％ CI =0.30-0.99,P ＜ 0.05) risk reduction in gastric cancer patients,the difference was statistical significance.Conclusion The number of T-bet positive lymphocyte in gastric carcinoma was positively correlated with patient' s survival,which supports the notion that the T-bet was a positive regulator,which played an important role in tumor micro-environment. Key words: Gastric carcinoma; T-bet; Transcription factor; Immunohistochemistry