Abstract
A Michelson interferometer is commonly used for evaluating the morphology of a cell. However, the interference imaging with reference and object beams is easily affected by external vibrations and environmental disturbances, leading to unstable interference patterns. In this paper, the three-dimensional surface morphology of the biological cell is evaluated by a new quantitative phase imaging method, which couples Michelson-like lateral shear interferometric microscopy with self-referencing numerical phase calibration. The Michelson-like lateral shear interferometric microscopy is constructed by replacing the two plane mirrors of the traditional Michelson interferometer with two common right-angle prisms and generates interference fringe patterns. The lateral shear is created and freely adjustable by simply translating/or rotating one right-angle prism. To calculate the phase information of the biological cells quantitatively, the classical Fourier transform method is used to process the recorded interferogram, and then the self-referencing numerical phase calibration method is utilized for acquiring accurate phase information. Successfully achieving quantitative phase imaging of a cell verifies the feasibility and practicability of the proposed method.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
